General description
Mass spectrometry (MS) compatible protease inhibitor cocktail and phosphatase inhibitor cocktail, with broad specificity for the inhibition of:
- Serine, cysteine and aspartic proteases, and metalloproteases
- Tyrosine, serine/threonine, acid and alkaline phosphatases
Specificity
Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases
Application
MS-SAFE Protease and Phosphatase Inhibitor has been used to measure ribosomal protein S6 kinase (S6K) activity.
Tested in mammalian cell lysates and liver tissue extracts. Designed for use in samples to be analyzed by mass spectrometry.
Features and Benefits
Comprehensive protease and phosphatase inhibitor cocktail for mass spectrometry analysis
Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment
Allows accurate measurement of protein activity and identification of phosphorylation sites.
Components
Protease inhibitors:
- Bestatin hydrochloride
- Leupeptin
- Phosphoramidon disodium salt
- Pepstatin A
- Elastatinal
- Aprotinin
- Nafamostat mesylate
- Antipain
- Okadaic acid
- Sodium fluoride
- Sodium orthovanadate
- Bromotetramisole oxalate
- β-lactose
- DL-leucine
Quantity
One vial makes 20 mL of 1× inhibitor cocktail working solution, using either water or extraction/lysis buffer. Alternatively, a 10× concentrated solution may be prepared by adding 2 mL of water or extraction/lysis buffer. This 10× solution may then be diluted 10-fold into extraction/lysis buffer as needed for a 1× working solution.
Physical form
Lyophilized powder that is water-soluble
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41141606
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MSSAFE-5VL
- Temperature Control Device:
- Yes