General description
Note: 1 mU = 1 milliunit.
Recombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). Degrades fibrillar collagen Types I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13 resulting in enhanced proteolysis of the matrix for cell migration and proliferation.
Recombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). The catalytic domain is comprised of amino acid residues Tyr89 to Gly265 of mature human MT1-MMP. Hydrolyzes fibrillar collagens I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13, resulting in enhanced proteolysis of the matrix for cell migration and proliferation.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Amano, T., et al. 2005. Cell Res.15, 150.
Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
Sato, H., et al. 1994. Nature370, 61.
Knight, C.G., et al. 1992. REBS Lett.296, 263.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Assay: ≥. 90% (SDS-PAGE). Quality Level: 100. form: liquid. specific activity: ≥. 140 . mU/mg protein. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352200
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 475935-10UG