MTP(TM) Taq DNA Polymerase (C15-1355-875)

General description

MTP^™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5′→3′ DNA polymerase and exonuclease activities, is ∼95 kDa by SDS-PAGE, and has no detectable endonuclease or 3′→5′ exonuclease activities. Each lot of MTP Taq undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA.

Contaminating DNA present in most other polymerase preparations often preclude or obscure the accurate interpretation of results, especially when targeting conserved sequences, e.g., bacterial 16S rRNA region. Through Sigma′s proprietary DNA removal methods and strict quality control standards, we can ensure the absence of the most commonly found contaminant DNA. Each lot of MTP Taq is assayed using PCR and primers specific to the conserved region of bacterial 16S rRNA, the Taq expression vector, and the human β-actin gene.

While MTP Taq ensures a high-quality, low contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10× MTP Taq Buffer with each tube of MTP Taq DNA Polymerase. Each lot of 10× MTP Taq Buffer undergoes the same strict quality control testing as MTP Taq DNA polymerase to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

Application

MTP^™ Taq DNA Polymerase has been used:

• for the amplification of bacterial 16S rRNA genes from purified DNA
• bacterial genome analysis
• pathogen detection

Biochem/physiol Actions

MTP^™ Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands.

Features and Benefits

• Low contaminant DNA polymerase
• Prevents false positive PCR results from contaminating bacterial DNA

Components

• MTP Taq DNA Polymerase (D7067)
• 10x MTP Taq Buffer (M9943)

Unit Definition

One unit incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74 °C.

Other Notes

Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.

Store at –20 °C. For convenience, 10x MTP Taq Buffer can be stored at room temperature.

View more detailed information on MTP Taq DNA Polymerase at www.sigma-aldrich.com/mtptaq.

Legal Information

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

MTP is a trademark of Sigma-Aldrich Co. LLC