MUG Brilliant Green Bile Broth, 5 kg

Brilliant Green Bile Broth is one of the most widely used medium for the detection of coliform bacteria in water, wastewater, foods, and milk and dairy products. This medium is formulated as per APHA (1, 2, 3) for the presumptive identification and confirmation of coliform bacteria (4, 5). Pancreatic digest of gelatin serves as a source of essential nutrients. Lactose is the fermentable carbohydrate. Ox gall inhibits gram-positive bacteria whereas the gram-negative bacteria are inhibited by brilliant green. Production of gas from lactose fermentation is detected by incorporating inverted Durham's tube, which indicates the positive evidence of faecal coliform since non faecal coliforms growing in this medium do not produce gas. Gram-positive spore formers may produce gas if the bile or brilliant green inhibition is weakened by reaction with food material. The fluorogenic compound, MUG (4-Methylumbelliferyl-b-D-glucuronide) in the medium permits the rapid detection of E.coli which produces a blue fluorescence when hydrolyzed by the enzyme b-glucuronidase and is observed using a long-wave UV light source. During examination of water samples, growth from presumptive positive tubes showing gas in Lactose Broth (M026) or Lauryl Tryptose Broth (M080) is inoculated in Brilliant Green Bile Broth 2% (M121). Gas formation within 48 ± 2 hours confirms the presumptive test (1). Storage and Shelf-life: Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. References: 1. Greenberg A. E., Eaton A. D. and Clesceri L. S., (Eds.), 1998, Standard Methods for the Examination of Water and Wastewater, 20th ed., APHA, Washington, D.C. 2. Downes F. P. and Ito K. (Eds.) 2001, Compendium of Methods for the Microbiological Examination of Food. 4th Ed, APHA, Washington, D.C. 3. Richardson G., (Ed.), 1985, Standard Methods for the Examination of Dairy Products, 15th Ed, APHA, Washington, D.C. 4. McCrady and Langerin, 1932, J. Dairy Science, 15:321. 5. McCrady, 1937, Am. J. Publ. Health, 27:1243.