Mycoplasma Broth Base (PPLO Broth Base) without CV, 2.5KG

PPLO Broth Base w/o CV (Mycoplasma Broth Base w/o CV) with added enrichment supplement and horse serum or ascitic fluid is used for isolation and cultivation of Mycoplasma species (pleuropneumonia like organisms). This medium does not contain crystal violet. Most of the Mycoplasma species are aerobic or facultatively anaerobic but some are microaerophilic. Broth cultures on subculturing onto PPLO Agar, produce colonies exhibiting the typical morphology, i.e., fried egg appearance. Appearance: Cream to yellow homogeneous free flowing powder Color and Clarity of prepared medium: Yellow colored clear solution in tubes Reaction of 2.1% w/v aqueous solution at 25°C. pH : 7.8±0.2 pH : 7.8±0.2 Cultural characteristics observed in presence of 10% Carbon dioxide with added 1% Horse serum (RM1239) and Mycoplasma Enrichment Supplement(FD075), after an incubation at 22-35°C for 48 hours