MYSTICQ MICRORNA SYBR GREEN QPCR READYMIX LOW ROX (C005B-446240)

General description

The MystiCq^® microRNA SYBR^® Green qPCR ReadyMix^™ is one of the integral components of the MystiCq microRNA qPCR Assay System. This master mix is a specially formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for highly specific, reliable, and reproducible microRNA qPCR assays. Simply add the specific MystiCq microRNA qPCR Assay Primer, the MystiCq Universal PCR primer, and cDNA template generated by the MystiCq microRNA cDNA Synthesis Kit and you′re ready to go. Because there are different methods used for fluorescent normalization on different real-time PCR instruments, there is a MystiCq microRNA SYBR Green qPCR ReadyMix available for your platform.

Application

MystiCq microRNA SYBR Green qPCR ReadyMix, Low ROX^™, is compatible with the following platforms: Applied Biosystems 7500, Applied Biosystems 7500 Fast, Applied Biosystems ViiA 7, Stratagene Mx3000P^®, Stratagene Mx3005P^®, Stratagene Mx4000^™.

Features and Benefits

• Exclusively for use with the MystiCq microRNA qPCR Assay Primers, Control Primers, and Universal PCR primer
• 2X concentrated for easy reaction setup with hot-start Taq, dNTPs and optimized buffer
• Passive reference dye (if required) included at the optimal concentration
• This master mix can readily amplify fragments between 400 and 500 bp; however, for best results, amplicon size should be limited to 80 - 200 bp

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL

Legal Information

Mx3000P is a registered trademark of Agilent Technologies, Inc.

Mx3005P is a registered trademark of Agilent Technologies, Inc.

Mx4000 is a trademark of Agilent Technologies, Inc.

MystiCq is a registered trademark of Qiagen Beverly Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

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