General description
The MystiCq® microRNA SYBR® Green qPCR ReadyMix™ is one of the integral components of the MystiCq microRNA qPCR Assay System. This master mix is a specially formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for highly specific, reliable, and reproducible microRNA qPCR assays. Simply add the specific MystiCq microRNA qPCR Assay Primer, the MystiCq Universal PCR primer, and cDNA template generated by the MystiCq microRNA cDNA Synthesis Kit and you′re ready to go. Because there are different methods used for fluorescent normalization on different real-time PCR instruments, there is a MystiCq microRNA SYBR Green qPCR ReadyMix available for your platform.
Application
MystiCq microRNA SYBR Green qPCR ReadyMix is compatible with the following platforms: Bio-Rad CFX384™, Bio-Rad CFX96™, Bio-Rad MiniOpticon™, Bio-Rad/MJ Chromo4™, Bio-Rad/MJ Opticon 2, Bio-Rad/MJ Opticon, Cepheid SmartCycler®, Eppendorf Mastercycler® ep realplex, Eppendorf Mastercycler ep realplex2 s, Illumina Eco qPCR, Qiagen/Corbett Rotor-Gene® 3000, Qiagen/Corbett Rotor-Gene 6000, Qiagen/Corbett Rotor-Gene Q, Roche LightCycler® 480.
Features and Benefits
- Exclusively for use with the MystiCq microRNA qPCR Assay Primers, Control Primers, and Universal PCR primer
- 2X concentrated for easy reaction setup with hot-start Taq, dNTPs and optimized buffer
- Passive reference dye (if required) included at the optimal concentration
- This master mix can readily amplify fragments between 400 and 500 bp; however, for best results, amplicon size should be limited to 80 - 200 bp
Packaging
Default reaction volume is 50 μL
100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL
Legal Information
CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Chromo4 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
LightCycler is a registered trademark of Roche
Mastercycler is a registered trademark of Eppendorf AG
MystiCq is a registered trademark of Qiagen Beverly Inc.
Opticon is a trademark of Bio-Rad Laboratories, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
SYBR is a registered trademark of Life Technologies
SmartCycler is a registered trademark of Cepheid, Inc.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41181567
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MIRRM00-100RXN
- Temperature Control Device:
- Yes
