Application
Sequencing primers are used for double-stranded DNA sequencing of inserts in bacterial FLAG expression vectors.
The N-26 primer is used for sequencing DNA at the 5′ end of the ORF in E. coli FLAG® expression vectors. This corresponds to the amino-terminal fusion junction of FLAG fusion proteins. It can also be used to sequence the amino-terminus of carboxy-terminal FLAG fusion proteins. Complementary sequence is immediately 5′ to the tac promoter region.
Other Notes
(5′-CAT CAT AAC GGT TCT GGC AAA TAT TC-3′)
Legal Information
FLAG is a registered trademark of Sigma-Aldrich Co. LLC
- UPC:
- 41105601
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- P7832-1UG
- Product Size:
- 1/EA
