N-ACETYL-B-D-GLUCOS-AMINIDASE

General description

N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination of N-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.

Application

N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination of N-acetyl-β-D-glucosaminidase in urine in life-science research applications.

Packaging

1 kit containing 3 components

Preparation Note

Working solution: Preparation of solutions:

1. Buffer solution
   Dissolve the contents of bottle 1 with 55 ml double-distilledwater.
2. Substrate solution
   Dissolve the contents of bottle 2 with 55 ml solution I.
3. Stop reagent
   Dissolve the contents of bottle 3 with 110 ml double-distilled water.

Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.

Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.