Cell Line Origin
Mouse neuroblastoma
Cell Line Description
A 6-thioguanine-resistant clone of N4 (a clonal line produced from the mouse neuroblastoma C1300). (Mouse strain A/Jax). This cell line lacks any appreciable activity of either choline acetyltransferase or tyrosine hydroxylase enzymes.
Application
Used in the generation of cell hybrids.
Culture Medium
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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- UPC:
- 12352207
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 08062515-1VL
- Temperature Control Device:
- Yes
