General description
Nicotinamide adenine dinucleotide phosphate (NADP) and NADPH form a redox pair. The NADPH/NADP ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo.
Application
The β-NADH phosphate disodium salt has been used for the quantification of glucose in glycogen. It has also been used for the hexokinase assay.
Preparation Note
Storage conditions (working solution): 2 to 8 °C
10 mg/ml in aqueous solution, contains at least 99% NADP after 4 weeks at 2 to 8 °C
Analysis Note
Absorbance coefficients: 18.0 x 103 [l x mol-1 x cm-1] at 260 nm, 5.9 x 103 [l x mol-1 x cm-1] at 333 nm (CN complex)
Absorbance at 340 nm: Imidazol buffer, 100 mM pH 6.7, NADP 2 mM (= 14.87 mg/10 ml) : A340 approximately 0.100.
Absorbance at 360 nm: Aqueous solution of NADP, 10 mg/ml: A360 approximately 0.200-0.250.
Other Notes
For life science research only. Not for use in diagnostic procedures.
- UPC:
- 51372118
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 10128031001
- CAS:
- 24292-60-2
