NBT-BCIP(R) solution (C15-1308-277)

Physical form

solution of 18.8 mg/ml nitro-blue tetrazolium chloride; 9.4 mg/ml 5-bromo-4-chloro-3-indolylphosphate toluidine salt in 67% DMSO

Other Notes

Chromogenic phosphatase substrate producing a blue-colored precipitate at the site of enzymatic activity

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

grade: suitable as substrate for alkaline phosphatase in dot blots. Quality Level: 100. product line: BioReagent. form: liquid. solubility: 0.1 M Tris-HCl, pH 7.4: 0.0175 . mL/mL, clear. application(s): diagnostic assay manufacturing
hematology
histology. storage temp.: 2-8°C. SMILES string: [Cl-].[Cl-].Cc1ccc(N)cc1.OP(O)(: O)Oc2c[nH]c3ccc(Br)c(Cl)c23.COc4cc(ccc4-[n+]5nc(nn5-c6ccc(cc6)[N+]([O-]): O)-c7ccccc7)-c8ccc(c(OC)c8)-[n+]9nc(nn9-c%10ccc(cc%10)[N+]([O-]): O)-c%11ccccc%11. InChI: 1S/C40H30N10O6.C8H6BrClNO4P.C7H9N.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54. 9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14. 1-6-2-4-7(8)5-3-6. . /h3-26H,1-2H3. 1-3,11H,(H2,12,13,14). 2-5H,8H2,1H3. 2*1H/q+2. . . . /p-2. InChI key: GDPIIVBVMSORRQ-UHFFFAOYSA-L. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): 152.6 °F - closed cup. Flash Point(C): 67 °C - closed cup. Personal Protective Equipment: Eyeshields, Gloves, type ABEK (EN14387) respirator filter.