Cell Line Origin
Human small cell lung cancer, drug-resistant
Cell Line Description
NCI-H69 VCR/R has been derived from the parent small cell lung cancer line NCI-H69 (Sigma Catalogue number. 91091802) It is recommended to culture cells without drug upon resuscitation; add vincristine once growth is fully established. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Application
Drug-resistance studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17
Culture Medium
RPMI 1640 + 2mM Glutamine + 2.0 μg/ml vincristine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x 100,000 cells/ml; 5% CO2; 37ºC. Resuscitate in drug-free medium, add after growth has been established.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: human lung. growth mode: Aggregates in suspension. karyotype: Not specified. morphology: Not specified. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51343403
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 96042330-1VL
