Cell Line Origin
Human Caucasian IgA-producing plasmacytoma
Cell Line Description
This IgA-producing cell line has been established from a malignant effusion in a 62-year-old Caucasian woman with myeloma. They are positive for plasma cell antigen 1 (PCA-1), transferrin receptor and CD38 but negative for early B cell developmental markers. HLA DR and CALLA. NCI-H929 is negative for Epstein-Barr virus nuclear antigen. A rearrangement of the c-myc proto-oncogene has been observed as well as the expression of c-myc RNA and an activated ras allele.
Application
Chemotherapeutic agent studies, receptor studies, carcinogenesis assays.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 9,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8,11
vWA: 14,15
Culture Medium
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 2-4 x 100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: human unknown/unspecified. growth mode: Suspension. karyotype: Near-tetraploid (8q+). morphology: Lymphoblastoid. products: Immunoglobulin A (IgA). receptors: Transferrin, CD38. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 12352207
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 95050415-1VL
