Cell Line Origin
Mouse neuroblastoma x Rat neurone hybrid
Cell Line Description
Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.
Application
Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.
Recommended culture medium: DMEM + 2 mM
Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x104 viable cells/cm2 at 37°C and 5% CO2. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: Mouse x Rat Hybridoma nerve. growth mode: Adherent. karyotype: Not specified. morphology: Neuronal. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 92090903-1VL
