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nd7/23 cells mouse neuroblastoma x rat neurone hybrid (c15-1312-962)

ND7/23 CELLS MOUSE NEUROBLASTOMA X RAT NEURONE HYBRID (C15-1312-962)

Sigma-Aldrich

Catalog No.
C15-1312-962
Manufacturer No.
92090903-1VL
Manufacturer Name
Sigma-Aldrich
Quantity
1
Unit of Measure
VI
Price: $1,181.14
List Price: $1,312.38

Cell Line Origin Mouse neuroblastoma x Rat neurone hybrid Cell Line Description Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion. Application Mouse neuroblastoma (N18 tg 2) and

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Cell Line Origin

Mouse neuroblastoma x Rat neurone hybrid

Cell Line Description

Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.

Application

Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.

Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS

Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x104 viable cells/cm2 at 37°C and 5% CO2. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source: Mouse x Rat Hybridoma nerve. growth mode: Adherent. karyotype: Not specified. morphology: Neuronal. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.
UPC:
41106514
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
92090903-1VL


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