General description
A cell-permeable Antennapedia-NBD mutated (Trp739 and Trp741 replaced with Ala) fusion peptide analog of NEMO-Binding Domain Binding Peptide (Cat. No. 480025) that serves as a negative control. Shown to be defective in binding to NEMO. Does not affect the TNF-α-induced phosphorylation of c-Jun and DNA binding of the transcription factor Oct-1.
A cell-permeable, Antennapedia-NBD mutated (Trp739 → Ala and Trp741 → Ala) fusion peptide analog of NEMO-Binding Domain Binding peptide (Cat. No. 480025) that serves as a negative control. Reported to be defective in binding to NEMO. Does not affect the TNF-α-induced phosphorylation of c-Jun or DNA binding of the transcription factor Oct-1.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Negative control defective in binding to NEMO
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Sequence
H₂N-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Ala-Ser-Ala-Leu-Gln-Thr-Glu-OH
Other Notes
Jimi, E., et al. 2004. Nat. Med., 10, 617.
May, M.J., et al. 2000. Science289, 1550.
This peptide will precipitate when added to tissue culture medium even at 100 µM concentrations; however, sufficient material will stay in solution to permeate cells.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Molecular Weight: 3462.98. Empirical Formula: C154H249N47O42S. Quality Level: 100. Assay: ≥. 95% (HPLC). form: lyophilized solid. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, desiccated (hygroscopic). color: off-white. solubility: DMSO: 50 . mg/mL. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352202
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 480030-500UG