Neuroredtm C2 neuron fluorescent probe

Product introduction：Nerve terminal probes are a series of cationic styryl fluorescent dyes used to track the synaptic activity of neuromuscular connections or synapses. Such dyes usually have lipophilic tails (two carbon chains) and highly hydrophilic heads with cations. The nerveredtm series of probes are dyes with three double bonds. Cationic styryl dyes function by activity dependent staining of synaptic vesicles. When the dye is co incubated with cells or tissues, the aqueous part of the dye has no fluorescence, while the lipophilic tail of the dye inserts into the cell membrane and exhibits strong fluorescence. After nerve stimulation, the dye is encapsulated in the vesicles during endocytosis. Therefore, after washing the dye attached to the cell surface, the fluorescence signal intensity indicates the number of newly formed vesicles. On the contrary, during exocytosis, the dye is released from the vesicles together with neurotransmitters, resulting in the reduction of fluorescence signal. Therefore, the change in fluorescence intensity reflects the situation of endocytosis / exocytosis or synaptic activity. The rate of fluorescence increase during endocytosis - "binding rate" and the rate of fluorescence decrease during exocytosis - "dissociation rate" vary according to the dye type. In general, dyes with longer lipophilic tails and more double bonds have higher affinity for membranes and thus higher binding rates and lower dissociation rates. This product is equivalent to FM4-64. Nerveredtm C2 dye cannot be used for fixed cell staining. Nm series dyes can be selected for fixed cell staining.Product parameters：Appearance: Purple solid soluble in water ex/em:543/- nm (emission in MeOH is too weak to measure) ex/em:510/750 nm (in membranes) molecular formula: c30h45br2n3 molecular weight: 607.5Scope of application：Neurofluorescent dye.