Neutral Protease AF From Clostridium Histolyticum

DescriptionNeutral Protease AF is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This purified metalloprotease is suitable for the dissociation of tissue in clinical applications. For especially gentle cell isolation it is recommended to use Neutral Protease AF in combination with highly purified Collagenase AF-1  SpecificationsPlant-based fermentation process. Contains chromatographically purified neutral protease.Vial contains not less than 100 DMC U of neutral protease.Neutral protease activity (DMC) ≥ 0.50 U/mg.Endotoxin: ≤ 100 EU/mg (Ph. Eur.)Largely free of collagenolytic activity.Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of ClostridiaEndproduct is 0.22 µm filtered. References and DefinitionsUnit definition:Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmol peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)]. References1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93. Specifications and Purity: ≥ 0.50 U/mg. MDL Number: MFCD02253021. EC Number: 232-642-4. PubChem CID: 87574717. Isomeric SMILES: CCC1=C(C=CC(=C1)C(F)(F)F)C(=O)NC2(CCCN(C2)C)C3=CC=NC=C3.CN1CCCC(C1)(C2=CN=CC=C2)NC(=O)C3=C(C=C(C=C3SC)C(F)(F)F)OC.Cl. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/N278967-SCI_bf4e24cb346cec659c3db07939959684.pdf.