DescriptionNeutral Protease NB is chromatographically purified and contains an increased proteolytic activity. It is largely free from collagenolytic activity (PZ U) and the endotoxin level is very low.ApplicationNeutral Protease NB is, mostly in combination with Collagenase NB 1 or another Collagenase NB product, suitable for cell isolation from various tissue types.SpecificationsContains highly active, chromatographically purified neutral protease from Clostridium histolyticum.Neutral protease activity (DMC): ≥ 4.00 U/mg.Largely free of collagenolytic activity.Endotoxin: ≤ 50 EU**/mg (Ph. Eur.)Mr ca. 34 000 • CAS [9001-92-7]References and DefinitionsUnit definition: *Neutral Protease: 1 DMC Unit catalyzes the cleavage of 1 µmole peptide bond from dimethylcasein per minute at 25 °C, pH 7.0, expressed in terms of newly formed terminal amino groups, determined with TNBS (1).**Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)). References1. Lin, Y.-C. et al. (1969) J. Biol. Chem. 244, 789-93. Specifications and Purity: High Active Grade,≥ 4.00 U/mg.. MDL Number: MFCD00132092. EC Number: 232-642-4. PubChem CID: 87574717. Isomeric SMILES: CCC1=C(C=CC(=C1)C(F)(F)F)C(=O)NC2(CCCN(C2)C)C3=CC=NC=C3.CN1CCCC(C1)(C2=CN=CC=C2)NC(=O)C3=C(C=C(C=C3SC)C(F)(F)F)OC.Cl.
- UPC:
- 12352204
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 0.96 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- N279017-80U
- CAS:
- 9001-92-7
- Product Size:
- 80U
