NeutrAvidin Protein is a specially-prepared form of avidin that decreases background in biotin-binding.Features include:• Near-neutral isoelectric point—pI = 6.3, more neutral than native avidin• Nearly devoid of glycosylation—decreased possibility of lectin binding compared to native avidin• No RYD recognition sequence—no known off-target binding domains like streptavidin• Affordable—significantly less expensive than streptavidinNeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified NeutrAvidin Protein provides exceptional performance in western blot, ELISA, and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin.Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin-binding proteins. The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.
Specification: ExactAb™, Validated, Ex:565nm, Em:575nm, 1.0 mg/mL
Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/RP188520-SCI_bf89bc5f39dbd933c8de428860a2a682.pdf
- UPC:
- 12352200
- Condition:
- New
- Availability:
- 5-10 days
- Weight:
- 0.07 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- rp188520-10μg
- Product Size:
- 10μg
