Cell Line Origin
Mouse pre-B lymphoblast
Cell Line Description
Derived from a spontaneous lymphoma in an NFS.C58v-1 mouse. These pre-B cells are negative for surface Ig, Ia and ThB.
Culture Medium
DMEM + 10% NCTC 109 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 1mM Oxaloacetic acid + 50μM 2-Mercaptoethanol (2ME) + 2μg/ml Transferrin + 0.2IU/ml Insulin + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell
Other Notes
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biological source: mouse blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblast. products: Not specified. receptors: Ly-17, Lyb-2, Ly-1, Ly-5 (B220). technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51201516
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 93090713-1VL
- Temperature Control Device:
- Yes
