Cell Line Origin
Mouse pre-B lymphoblast
Cell Line Description
Established from a lymphoma produced in an NFS/N mouse by injecting CaS-25M ecotropic MuLV. Cells show markers of large pre-B lymphoblasts and spontaneously produce ecotropic and mink cell focus-forming viruses.
Application
Virus studies: MuLV, ecotropic and focus-forming viruses
Culture Medium
DMEM + 2mM Glutamine + 1mM Oxaloacetic acid + 0.2U/ml Bovine Insulin + 2ug/ml Transferrin + 50μM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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biological source: mouse blood. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblast. products: Ecotropic and mink cell focus-forming viruses. receptors: Not specified. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 41106514
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 88041904-1VL