NHS-Phosphine

Product introduction:NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.Features of NHS-Phosphine:• :Soluble:—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples:• :Compatible:—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents:• :Specific:—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides):• :Chemoselective:—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency:This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine group (PPh2) reacts with azide-labeled molecules by a mechanism known asStaudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, this NHS-Phosphine reagent facilitates a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.• :Reactive Moiety:NHS Ester,Phosphine :• :Crosslinker Type:Heterobifunctional :• :Form:Powder:• :Spacer:Short(<10 Å) :• :Labeling Method:Chemical Labeling :• :Spacer Arm Length:5.4 Å. Molecular Weight: 461.4