General description
Linearity
The method is linear in the range from 0.02 mg nitrite or nitrate/l sample solution to 5.00 mg nitrite or nitrate/l sample solution.
Detection limit: 0.02mg/l for nitrite and nitrate
Specificity
Under the provided conditions, nitrate reductase reacts specifically with nitrate ions.
Application
Semimicro method for the determination of nitrite and/or nitrate to investigate water, waste water, environmental samples, plant material, foodstuffs, drugs, cosmetics, and biological samples. Nitrite/Nitrate, Colorimetric Test was used in a study done to estimate the in vivo effects of myrtle oil (Myrtii oleum) on the antioxidant enzymes such as catalase and superoxide dismutase in alloxan and normoglycaemic diabetic rabbits.
Packaging
1 test combination containing 5 components.
Preparation Note
Working solution: Preparation of the solutions
- Use the contents of bottle #1 undiluted, bring to 15 to 25 °C before use.
- Place one Coenzyme-tablet in a beaker and dissolve in 3 ml of the solution from bottle 1. Remove the Coenzyme-tablet using the tweezers provided. The resulting solution is reaction mixture 2 and is sufficient for 12 nitrate determinations, bring to 15 to 25 °C before use.
- Dissolve the contents of one bottle #3 in 0.7 ml of double-dist. water to give solution 3.
- Use the contents of one bottle #4 undiluted.
- Use the contents of one bottle #5 undiluted.
Storage conditions (working solution):
- Prepare Reaction Mixture 2 immediately before use.
- Solution of Nitrate reductase is stable at 2 to 8 °C for 2 weeks.
- Solution of Color reagent I is stable at 2 to 8 °C for 3 months.
- Solution of Color reagent II is stable at 2 to 8 °C for 3 months.
Other Notes
For life science research only. Not for use in diagnostic procedures.
- UPC:
- 41141520
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 11746081001
