Normal Chicken IgY Antibody, purified | AC146 (C15-1318-092)

General description

Chickens transfer high quantities of IgY (IgG) into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One week′s eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding. However, there are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian complement component C1 and it does not perform as a precipitating antibody using standard solutions.

Application

Gel Electrophoresis Analysis: 20 µg of reduced Chicken IgY from a representative lot was resolved by SDS-PAGE and visualized using total protein stain.

Note: To be used as a negative control for ChIP Ab+ MeCP2, Cat. No. 17-10491.

Note: For use as a negative control, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction.

Research Category
Secondary & Control Antibodies

Research Sub Category
Isotype Control Antibodies

Quality

Evaluated by Western Blotting using non-reduced Chicken IgY.

Western Blotting Analysis: 0.01 µg of this non-reduced Chicken IgY was detected by a Rabbit anti-Chicken secondary antibody.

Target description

~180 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified

Purified chicken polyclonal in buffer containing PBS with 0.05% sodium azide.

T-gel

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: chicken. Quality Level: 100. antibody form: purified antibody. clone: polyclonal. manufacturer/tradename: Chemicon^®. . concentration: 1 . mg/mL. technique(s): blocking: suitable (interfering antibodies). isotype: IgY. shipped in: wet ice. target post-translational modification: unmodified. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.