General description
Recombinant, human neuregulin 1-β1/heregulin 1-β1 extracellular domain (NRG1-beta1/HRG1-beta1 ECD) consisting of amino acids 2-246 and expressed in E. coli. All NRG1 isoforms contain an EGF-like domain (β-splice variant) that is used to directly bind to the ErbB3 or ErbB4 receptor tyrosine kinases.
Biochem/physiol Actions
ED₅₀ = 2.5-12.5 ng/ml as measured in a serum-free proliferation assay using MCF7 human breast adenocarcinoma cells.
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 0.2 µm filtered acetonitrile, TFA, BSA.
Reconstitution
Following reconstitution refrigerate (4°C) for short term-storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute 50 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Other Notes
Buonanno, A., and G.D Fischbach 2001. Curr. Opin. Neurobiol.11, 287.
Adlkofer, K. and C. Lai 2000. Glia29, 104.
Garratt, A.N. et al., 2000. BioEssays22, 987.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Assay: ≥. 90% (SDS-PAGE). Quality Level: 100. form: lyophilized. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze. impurities: ≤. 1.0 . EU/μ. g protein Endotoxin. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3.- UPC:
- 12352200
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 492028-50UG
