Storage buffer: 10mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 200µg/ml Recombinant Albumin, 50% Glycerol, pH 7.4NruI can recognize the TCG/CGA palindromic sequence, and form a flat terminal after enzyme digestion. The isozymes of NRUI include Bsp68I, BtuMI and RruI. Recombinant albumin was added to both NruI and 10×Cut Reaction Buffer to ensure the safety and stability of the product.Product compositionL398135Component1 KU5 KUStorageL398135ANruI50 μL250 μL-20°C. Avoid freeze/thaw cycleL398135B10×Cut Reaction Buffer 400 μL2 mL-20°C. Avoid freeze/thaw cycleSourceE.coliRestriction Enzyme CuttingSite5’…TCG↓CGA…3’3’…AGC↑GCT…5’ Enzyme Activity DefinitionIn a total reaction system of 50 μl, the amount of enzyme required for enzyme digestion of 1 μg λDNA within 1 h at 37℃ was defined as one active single site (U).Protocol(1) Configure the reaction system, as shown in the following tableComponentvolumeRNase-free WaterTo 50 μl10×Cut Reaction Buffer5 μlDNA1 μgNruI1 μl* (2) Gently mix, do not oscillate the vortex, and then instantaneous centrifugation.(3) Incubation at 37°C for 30 min.Note: *Nrul is finally added to the system, generally 50 μl reaction system to add 1μl of Nrul, can also be adjusted according to the experiment, it is recommended that the amount of enzyme added does not exceed 10% of the total system to avoid star activity.Cautions(1) Methylation sensitivity of Nrul:Dam methylation: Methylation and cleavage sites may overlap resulting in impaired cleavageDcm methylation: insensitiveCpG methylation: Blocking cleavage(2) Before the Meche reaction, try to ensure that the DNA does not contain impurities such as phenol, chloroform, alcohol, EDTA, so as not to affect the effect of enzyme digestion.(3) This product is for scientific research only and shall not be used for other purposes.
Specifictaions: EnzymoPure™, BioReagent, for DNA synthesis, Suitable for molecular biology, PCR Reagent, For In Vitro Transcription, for DNA and RNA applications, 20 U/μl
Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/L398135-SCI_deb563da64e9e22d44e22298b52663ec.pdf
- UPC:
- 51291804
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 7.06 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- L398135-1KU
- Product Size:
- 1KU
