Cell Line Origin
Mouse neuroblastoma
Cell Line Description
A bromodeoxyuridine-resistant mutant of NS20Y (Sigma Catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 Mouse strain A/Jax.
Application
Used in the generation of cell hybrids.
Culture Medium
DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
- UPC:
- 51293600
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 08062510-1VL
- Product Size:
- 1VL
