NSP-SA-NHS (C09-1157-247)

NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed.In VitroNSP-SA-NHS marking?/n(1) 1mg BSA was dissolved in 0.3 mL PBS (solution A), and 0.2 mg NSP-SA-NHS was dissolved in 40 μL DMF (solution B). ?/n(2) Add solution B to solution A, mix at 18°C, 130 rpm, and dark for 12 h. ?/n(3) The mixture was dialyzed with 500 mL of distilled water at 4°C for 2 d, during which distilled water was changed twice (solution C). ?/n(4) Slowly add solution B to solution C, mix at 18°C, 130 rpm, and dark for 24 h, and then dialysis with distilled water for 48 h. During this period, change distilled water four times. ?/n(5) Dilute the coupling compound with distilled water to a suitable concentration, and analyze it with an ultraviolet spectrophotometer. MCE has not independently confirmed the accuracy of these methods. They are for reference only.Form:Solid. Specifications and Purity: 99%. Molecular Formula: C32H31N3O10S2. Molecular Weight: 681.73. PubChem CID: 59642932. Isomeric SMILES: CC1=CC=C(C=C1)S(=O)(=O)N(CCCC(=O)ON2C(=O)CCC2=O)C(=O)C3=C4C=CC=CC4=[N+](C5=CC=CC=C53)CCCS(=O)(=O)[O-].