NuSieve 3:1 AGAROSE 500G

NuSieve TM 3:1 Agarose is a molecular biology grade, standard melting temperature agarose that yields strong gels for fine resolution of small DNA, RNA, and PCR products ≤ 1 kb. NuSieve TM 3:1 Agarose is designed for analytical electrophoresis rather than in gel reactions or downstream applications. These easy-to-handle gels enhance the speed of gel processing and blotting. Performance testing of NuSieve TM 3:1 Agarose ensures fine resolution of DNA fragments up to 1,000 bp, though this agarose is capable of finely resolving DNA fragments ranging from 10 bp up to 1,500 bp. Benefits: All Lonza Agarose is quality tested to certify performance High gel strength Exceptional resolution of 50 bp to 1000 bp DNA fragments Standard melting temperature Applications: Fine/Small Fragment Electrophoreisis Analytical Specifications: Gelling temperature (4%)  32.5 - 38.0°C Melting temperature (4%)  ≤90°C Gel strength (4%)  ≥1,400 g/cm² PCR Products on a NuSieve™ 3:1 Agarose Gel – A 550 bp sequence from lambda DNA was amplified (25 cycles) using primers and Taq DNA polymerase supplied in the GeneAmp® kit (Roche Molecular Systems). PCR products and controls were electro phoresed on a 4% NuSieve™ 3:1 agarose gel in TAE buffer at 5 V/cm for 3 hours. Lane 1, Msp I digest of pBR322 DNA (1.5 μg); lane 2, Hae III digest of øX174 DNA (1.5 μg); lane 3, no DNA control; lanes 4 – 9, PCR products resulting from different reaction conditions (7 μl of 100 μl reaction mixture); and lane 10, a positive control where kit template was added.