Nutrient Agar with 1% Peptone, 500 g

Nutrient Agar with 1% Peptone has almost double concentration of the nitrogen sources than that used in Nutrient Agar, making it more nutritive. Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (1, 2). It is one of the several non-selective media useful in routine cultivation of microorganisms (3, 4). Beef extract and peptic digest of animal tissue provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients to non-fastidious organisms like Bacillus subtilis and Staphylococcus aureus . Sodium chloride maintains osmotic equilibrium of the medium. With the addition of 10% v/v blood or other biological fluids like ascetic fluid, serum etc, this media is recommended for growing fastidious organisms. Peptic digest of animal tissue and beef extract are nutritionally rich in supplying essential nitrogen and growth factors (5). Storage and Shelf-life: Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. References: 1.Lapage S., Shelton J. and Mitchell T., 1970, Methods in Microbiology', Norris J. and Ribbons D., (Eds.), Vol. 3A, Academic Press, London. 2. MacFaddin J. F., 2000, Biochemical Tests for Identification of Medical Bacteria, 3rd Ed., Lippincott, Williams and Wilkins, Baltimore. 3. Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., American Public Health Association, Washington, D.C. 4. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington, D.C. 5. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, Vol. 1, Williams and Wilkins, Baltimore