Compared to other basal media, Ham’s F-12 contains a wider variety of components, and no proteins or growth factors. Without hypoxanthine and L-glutamine Ham’s F-12 uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH. Applications Ham’s F-12 Nutrient Mixture without Hypoxanthine is useful for serum free clonal growth of Chinese Hamster Ovary (CHO) cells, chick embryos and mouse L-cells. Ham’s F-12 Nutrient Mixture without Hypoxanthine can also be used to culture myeloma cells and hybridomas, as well as primary rat hepatocytes and prostate epithelial cells. Recommendation Add L-glutamine prior to use at a final concentration of 1.0 mM. Supplement 10% FBS because F-12 contains no proteins or growth factors.
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- UPC:
- 51131509
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 112-153-131
- Temperature Control Device:
- Yes
