NZCYM HiVeg Medium, 500 g

In 1977 Blattner and colleagues developed a rich growth medium for the cultivation of recombinants strains of E. coli and propagation of bacteriophages (1). This medium provides amino acids, vitamins and other elements required for the growth of the cells (2). Soya peptone and HiVeg acid hydrolysate serve as a source of essential nutrients. Yeast extract serves as a source of B-Complex nutrients. Magnesium sulphate is a source of magnesium ions and is required in various enzymatic reactions including DNA replication (3). It also helps in the absorption of the phage to cells. Sodium chloride maintains the osmotic balance of the medium. NZCYM HiVeg Growth Medium is prepared by using vegetable based peptone in place of animal based peptones, making the medium BSE/TSE risks free. This medium is used for propagation of lambda and filamentous phage. For optimal binding of phage to cells, 0.2% maltose (10 ml of 20% solution/litre) is added to induce the lambda receptor (LamB) on the host cells. Maltose induction of the lambda receptor is not recommended when making liquid lysate phage stocks because liberated phage will bind to membrane fragments containing a high concentration of phage receptors. Appearance of Powder: Light yellow coloured, may have slight greenish tinge, homogeneous, free flowing powder. Color and Clarity of prepared medium: Light amber coloured, clear solution without any precipitate. Reaction: Reaction of 2.2% w/v aqueous solution is pH 7.0 ± 0.2 at 25oC Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours. Organisms (ATCC) Growth Escherichia coli (DH5 ) good-luxuriant Storage and Shelf-life: Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label. References: 1. Blattner, F. R., B. G. Williams, A. E. et al. 1977. Charon phages: Safer derivatives of bacteriophage for DNA cloning. Science 196:161. 2. Ausubel, F. M., R. Brent et al. 1994. Current protocols in molecular biology, vol. 1. Current Protocols, New York, NY. 3. Sambrook J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.