o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.Application:o-Phenylenediamine dihydrochloride has been used as a stop solution for evaluating bovine serum albumin (BSA) antibodies via enzyme-linked immunosorbent assay (ELISA). It has also been used to measure specific antibodies in pig serum by ELISA.
Specification: tablet, 60 mg substrate per tablet
MDL Number: MFCD00012966
Molecular Formula: C6H8N2·2HCl
Molecular Weight: 181.06
EC Number: 210-418-7
PubChem CID: 11990
Isomeric SMILES: C1=CC=C(C(=C1)N)N.Cl.Cl
Beilstein Registry Number: 3912045
- UPC:
- 51161728
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 0.07 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- O755378-100tabs
- CAS:
- 615-28-1
- Product Size:
- 100tabs
- Hazard Statement Codes:
- H410:H332:H400:H312:H317:H351:H341:H301:H319
