OACP4 C

Cell Line Description

OACP4 C was established from a primary tumour located in gastric cardia of a 55 year-old Caucasian male. The patient showed lymph node involvement with pleural metastases. OACP4 C was found to be tumourigenic in NMRI (female nude mice or NOD-SCID mice. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino, et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer.

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 12
D5S818: 9
D7S820: 9,11
THO1: 9
TPOX: 11
vWA: 16

Culture Medium

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 10% FBS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) up to 1:5 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Citation

If use of this culture results in a scientific publication, it should be cited in the publication as: OACP4 C (ECACC 11012005)