Osteopontin, His.Tag, Mouse, Recombinant, Mouse (C15-1304-070)

General description

M.W. 65,000 and 30,000 under reducing conditions.

Recombinant, mouse osteopontin fused at the C-terminus to a His•Tag sequence and expressed in NSO-derived Leu¹⁷-Asn²⁹⁴ (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca²⁺-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.

Recombinant, mouse osteopontin fused at the C-terminus to a His•Tag sequence and expressed in NSO-derived Leu¹⁷-Asn²⁹⁴ (Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.

Biochem/physiol Actions

~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 10⁵ cells/well

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.

Reconstitute to ≥100 µg/ml with sterile PBS.

Other Notes

Xie, Y., et al. 2001. Kidney Int.60, 1645.
Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.
Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Assay: ≥. 95% (SDS-PAGE). Quality Level: 100. form: lyophilized. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. impurities: ≤. 100 . pg/μ. g Endotoxins (pg/μ. g rmOPN). shipped in: ambient. storage temp.: −. 70°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.