OVCAR-3 HUMAN CANCER CELL LINE

General description

Ovarian carcinoma is a prevalent worldwide disease, with over 200,000 new cases diagnosed per year (1). The first-line chemotherapy for newly-diagnosed ovarian carcinoma is cisplatin, but despite high initial efficacy, the most common types of ovarian carcinomas frequently relapse into platinum-resistant forms and many demonstrate multi-drug resistance (1). The availability of cellular models that recapitulate both resistant and susceptible forms of the disease are essential to understanding drug resistance and advancing new options for treatment.

OVCAR-3 is a high-grade serous ovarian adenocarcinoma cell line established from a patient refractory to cisplatin, however the cell line remains sensitive to a variety of chemotherapeutic drugs (2). OVCAR-3 cells demonstrate increased glutathione content (3) and express the Wilms Tumor 1 protein (WT1), a marker of advanced serous ovarian carcinoma (4). OVCAR-3 cells are characterized by migration/invasion ability and are tumorigenic in nude mice (5). The OVCAR-3 cell line is well-established and one of the most highly-cited model systems for ovarian carcinoma.

Source:
OVCAR-3 was established from ascitic fluid from a 60-year-old patient with ovarian cancer refractory to cisplatin treatment (2).

References:
1. Nat Rev Disease Primers 2016; 2:16061.
2. Cancer Res 1997; 57:850—856.
3. Proc Natl Acad Sci USA 1992; 89:3070-3074.
4. BMC Cancer 2006; 6:90.
5. Gynecol Oncol 2015;138(2):372-377.

Application

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

• Each vial contains >=1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.