Overnight Express Autoinduction System 1 - Novagen (C15-1308-159)

General description

The Overnight Express^™ Autoinduction System allows the induction of protein expression without monitoring cell density and without conventional induction with isopropyl β-D-1-thiogalactopyranoside (IPTG). This system is extremely convenient for routine expression of proteins in multiple cultures and is ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones. The Overnight Express Autoinduction System together with the Novagen RoboPop Solubility Screening Kit and RoboPop Purification Kits allows rapid optimization of the host-vector combinations, expression conditions, and purification parameters for high-throughput production of proteins for structural or functional analysis.

Application

Overnight Express^™ Autoinduction System 1 - Novagen has been used for the purification of ¹⁵N-human prion proteins (HuPrPs). It is suitable for most routine recombinant protein expression applications.

Features and Benefits

• No need to monitor cell growth or add IPTG
• High cell densities and protein expression levels
• Increased soluble protein yield
• Induction of numerous expression clones simultaneously
• Usable with any conventional glucose-free bacterial growth medium
• Ideal for pET Expression System or other IPTG-inducible bacterial systems
• Compatible with cultures grown in flasks, culture tubes, and deep-well plates
• Minimal sample handling

Components

The Overnight Express^™ Autoinduction System 1 kit contains three concentrated sterile solutions:

• OnEx Solution 1 A blend of carbon sources optimized for tightly regulated uninduced growth to high cell density, followed by high-level induction
• OnEx Solution 2 A concentrated buffer and nitrogen-source bend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis
• OnEx Solution 3 Provides magnesium for maximal cell density

Warning

Toxicity: Standard Handling (A)

Other Notes

Grabski, A., et al. 2003. inNovations 17, 3.

Legal Information

Express is a trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level: 200. form: liquid. manufacturer/tradename: Novagen^®. . storage condition: OK to freeze. technique(s): protein expression: suitable, protein purification: suitable. storage temp.: 10-30°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.