Cell Line Origin
Mouse NS0/U x Mouse BALB/c spleen
Cell Line Description
The antibody recognises a rat macrophage cell surface antigen and labels all peritoneal and 90% of alveolar macrophages. Splenic red pulp macrophages are antigen +ve, marginal zone macrophages antigen -ve. The antibody also reacts with the 120kDa antigen on granulocytes and dendritic cells and recognises rat SIRP (personal communication).
Immunogen
Resident peritoneal cells from (PVG-RT1c x PVG RT1u)F1 and (PVG-RT1c x DA-RT1a)F1 rats
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: NS0/U. Reactivity: Anti-Macrophage subset, rat. technique(s): cell culture | mammalian: suitable. isotype: IgG2a. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51203604
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 88051304-1VL
