Cell Line Origin
Mouse NS0/1 x Mouse BALB/c spleen
Cell Line Description
The antibody recognises a 45kDa glycoprotein expressed on all haemopoietic and endothelial cells. The antigen is identical to mouse BCM1 antigen. The antibody inhibits allogeneic mixed lymphocyte reactions using lymph node cells as responders and spleen cells as stimulators.
Immunogen
Rat T blasts prepared by stimulating purified T helper cells with allogeneic irradiated rat spleen cells
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: NS0/1. Reactivity: Anti-BCM1, rat. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 12352207
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 86100910-1VL
- Temperature Control Device:
- Yes