The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.Application:p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Specification: liquid
MDL Number: MFCD00007319
Molecular Formula: C6H4NNa2O6P
Molecular Weight: 263.06
PubChem CID: 77949
Isomeric SMILES: C1=CC(=CC=C1[N+](=O)[O-])OP(=O)([O-])[O-].[Na+].[Na+]
- UPC:
- 51282812
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 0.62 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- P755472-100ml
- CAS:
- 4264-83-9
- Product Size:
- 100ml
- Hazard Statement Codes:
- H290:H318:H302:H373:H315