Cell Line Origin
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Cell Line Description
The hybridoma cell line P-tyr-1 was produced by fusing P3X63Ag8.653 mouse myeloma cells with spleen cells from a female BALB/c mouse immunised with aminobenzyl phosphonate. Aminobenzyl phosphonate is a structural analogue of the side-chain in phosphotyrosine, and was coupled to hemocyanin (antibody P-HC) for immunisation. The secreted IgG1 antibodies react with phosphotyrosine and will detect phosphotyrosine-containing proteins by immunoprecipitation and Western blot analysis. The antibody does not react with phosphoserine or phosphothreonine. P-tyr-1 was tested for Bovine Viral Diarrhoea Virus (BVDV) at ECACC and found to be negative.
Immunogen
Aminobenzyl phosphonate
Application
Detection of phosphotyrosine-containing proteins
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
biological source: Mouse x Mouse Hybridoma. ImmunoDon: BALB/c mouse spleen. Myeloma: P3X63Ag8.653. Reactivity: Anti-Phosphotyrosine. technique(s): cell culture | mammalian: suitable. isotype: IgG1. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51181616
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 95092903-1VL
