p75NTR Signaling Inhibitor, Cell-permeable, TAT-Pep5 - Calbiochem (C15-1304-390)

General description

The p75^NTR inhibitor Pep5 (Cat. No. 506180) is made cell-permeable by fusing it with the N-terminal protein transduction domain sequence (11 amino acids) from HIV protein TAT. Shown to act as an effective blocker of MAG- and Nogo-induced inhibition of neurite outgrowth in both DRG (dorsal root ganglion) and postnatal cerebellar neurons. A potential therapeutic agent against inhibition of regeneration in the central nervous system.

The p75^NTR inhibitor Pep5 (Cat. No. 506180) fused to the N-terminal, 11-amino acid protein transduction domain sequence from the HIV TAT protein for membrane permeability. Show to inhibit the association of p75^NTR with Rho-GDI in vitro. Reported to act as an effective blocker of MAG- and Nogo-induced inhibition of neurite outgrowth in dorsal root ganglion (DRG) and postnatal cerebellar neurons at a concentration of 100 nM. Reported to be a potential therapeutic agent for the inhibition of regeneration in the central nervous system.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Blocker of MAG- and Nogo-induced inhibition of neurite outgrowth

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H₂N-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Cys¹²-Phe-Phe-Arg-Gly-Gly-Phe-Phe-Asn-His-Asn-Pro-Arg-Tyr-Cys²⁶-OH

disulfide bond (12-26)

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Yamashita, T., and Tohyama, M. 2003. Nat. Neurosci.6, 461.
Schwarze, S.R., et al. 1999. Science285, 1569.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 3404.90. Empirical Formula: C₁₅₀H₂₂₇N₅₇O₃₂S₂. Quality Level: 100. Assay: ≥. 95% (HPLC). form: lyophilized solid. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, desiccated (hygroscopic), protect from light. solubility: DMSO: 5 . mg/mL. shipped in: ambient. storage temp.: 2-8°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.