PALCAM LISTERIA-SELECTIVE AGAR (BASE)

General description

GranuCult^® PALCAM agar (Base) provides quantitative cultivation of Listeria monocytogenes and inhibits the accompanying Gram-negative and most of the Gram-positive bacteria. Polymyxin, acriflavin, ceftazidime, and lithium chloride result in the selectivity of the medium. L. monocytogenes break down the esculin in the medium to glucose and esculetin. Esculetin forms an olive-green to black complex with iron (III) ions, which stains the colonies of L. monocytogenes. Mannitol-positive accompanying bacteria such as Staphylococci grow as yellow colonies if they are not inhibited.

Application

GranuCult^® PALCAM agar (Base) is suitable for the detection and isolation of Listeria monocytogenes from feces, biological samples, foodstuffs, and heavily contaminated material from the environment.

Analysis Note

Base tested with PALCAM-Listeria selective supplement acc. to VAN NETTEN et.al. art. no. 1.12122.
Appearance (clearness): clear to slightly opalescent
Appearance (color): red
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)):
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)):
Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 50 %
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 50 %
Recovery on test medium (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 50 %
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth(Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): partial inhibition
Black zone (Listeria monocytogenes ATCC 35152 (WDCM 00109)): black zone
Black zone (Listeria monocytogenes ATCC 13932 (WDCM 00021)): black zone
Black zone (Listeria innocua ATCC 33090 (WDCM 00017)): black zone
Incubation: up to 48 hours at 35 ± 1 °C, aerobic
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany