PANSORBIN Cells (C15-1304-444)

General description

PANSORBIN Cells are heat-killed, formalin-fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lyphocytes and for immunoprecipitation.

Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high affinity interaction of protein A with the Fc domain of IgG. PANSORBIN cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.

Warning

Toxicity: Standard Handling (A)

Physical form

Supplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2.

Other Notes

Kierszenbaum, F., et al. 1991. Immunology74, 317.
Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
Murakami, H., et al. 1988. Biochem. J.256, 917.
Kessler, S.W. 1975. J. Immunol. 115, 1617.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

PANSORBIN is a registered trademark of Merck KGaA, Darmstadt, Germany

form: suspension (Homogeneous). Quality Level: 100. manufacturer/tradename: Calbiochem^®. . storage condition: do not freeze. impurities: ≤. 2500 . cells/mL Viable cells. color: milky. capacity: ≥. 2.0 . mg(human IgG/100 mg cells). shipped in: ambient. storage temp.: 2-8°C. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H302. Precautionary Statements: P264 - P270 - P301 + P312 - P501. Hazard Classifications: Acute Tox. 4 Oral. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.