PDPH(3-(2-pyridyldithio)propionyl hydrazide)

Product introduction:PDPH is a short, pyridyldithiol-and-hydrazide crosslinker for reversibly conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).Features of PDPH :• :Reactive groups:pyridyldisulfide andhydrazide:• :Reactive toward: sulfhydryl groups and carbonyl (aldehyde) groups:• Short (9.2A), sulfhydryl-to-aldehyde crosslinker with disulfide bond spacer arm (cleavable):• Pyridyldithiol group results in attachment to sulfhydryls via disulfide bond, which can be cleaved with DTT, TCEP or other reducing agents:• Hydrazide group conjugates to oxidized sugars of glycoproteins and carbohydrates:• Usesodium meta-periodate to oxidize glycosylation (e.g., sialic acid) to reactive aldehyde groups:• Use withEDC to conjugate primary amine of hydrazide group to carboxyl groups:Product References:Crosslinker Application Guide -- search for recent literature references for this product:• :Reactive Moiety:Hydrazide,Pyridyldisulfide :• :Crosslinker Type:Heterobifunctional :• :Form:Powder:• :Spacer:Short(<10 Å),Cleavable:By Thiols :• :Labeling Method:Chemical Labeling :• :Spacer Arm Length:9.2 Å. Molecular Weight: 229.32