Cell Line Origin
Mouse embryo fibroblast, LRP-deficient, homozygous
Cell Line Description
The cell line PEA-13 is homozygous for a disruption of the gene for low density lipoprotein receptor-related protein (LRP). PEA-13 was derived from fibroblasts from day 15 C57BL/6x129 F1 hybrid mouse embryos. Pseudomonas exotoxin A (PEA) was used to select murine fibroblasts genetically deficient in LRP. Together with the cell lines PEA-10 (Sigma Catalogue number. 97040310) and MEF-1 (Sigma Catalogue number. 98061101) an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LRP-deficiency. This is of importance since LRP-deficient mouse embryos die early during gestation.
Application
Study of LRP and LRP deficiency.
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:6 to 1:10 i.e. seeding 2-4 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
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biological source: mouse embryo. growth mode: Adherent. karyotype: Not specified. morphology: Fibroblast. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 11131607
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 97040311-1VL
