PEA2

Cell Line Description

PEA2 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with a poorly differentiated adenocarcinoma. PEA2 was collected on relapse after treatment with cisplatin and prednimustine. PEA2 exhibits poor growth in semi-solid medium (agar). PEA2 is from the same patient as the PEA1 cell line (ECACC Catalogue number 10032306).

This cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) available at ECACC which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of estrogen protagonists.

Culture Medium

RPMI 1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time approximately 66 hours.

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.