Pectinase is a macerating enzyme comprised of pectintranseliminase, polygalacturonase, pectinesterase, xylanase, hemicellulases and cellulases. Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase causes random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. One unit will liberate one µmole of galacturonic acid from polygalacturonicacid per minute at pH 4.0 at 25°C. A concentration of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase), 24°C to 37°C for periods of 1 to 16 hours will yield good results for protoplasting.
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- UPC:
- 41106600
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 0219897925
- Model Number:
- C818Y11
- Temperature Control Device:
- Yes
