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PEPSIN FROM PORCINE GASTRIC MUCOSA (C005B-307132)

Catalog No.
C005B-307132
Mfr. No.
SRE0001-5G
Mfr. Name
Sigma-Aldrich
Qty/UOM
5
UOM
GM
Price: $1,380.87
List Price: $1,534.30

Application Pepsin cleavage can be used to produce F(ab′:) 2 fragments of antibodies. pepsin at www.

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Application

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Unit Definition

One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37° C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 Ã‚°C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Protein determined by E1%/280

Other Notes

View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.

Shipping Information:

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UPC:
51172468
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
SRE0001-5G
CAS:
9001-75-6
Temperature Control Device:
Yes

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